背景简介

WM266-4 is a metastatic human melanoma cell line with small flat mesenchymal morphology. This cell line was derived from the same patient as the cell lines WM115, WM239A, and WM165-1. WM115 cell line originated from the primary tumor, and WM266-4, WM165-1 and WM239A were from individual lymph-node metastases. The subject (55-year-old female) displayed VGP with a Clark level III tumor with thickness of 2.24mm.This cell line features the specific V600D (Val600Asp) mutation at codon 600 in the BRAF gene. This cell line also expresses PTEN loss of function including hemizygous deletion. WM266-4 cell line is wild type for N-RAS, c-KIT, and CDK4 genes. WM266-4 cells produce xenograft tumors when injected into immunocompromised mice.

保藏机构

Rockland; WM266-4-01-0001

基本信息

名称

人黑素瘤细胞WM-266-4(STR鉴定正确)

别称

WM-266-4; WM 266-4; WM-2664; WM2664; WM 2664; WM266; WM-266-mel; WM266mel; WC00097

种属

人

组织来源

皮肤

细胞类型

肿瘤细胞

疾病类型

黑色素瘤

生长特性

贴壁生长

细胞形态

上皮细胞样

冻存条件

冻存液：90%FBS，DMSO 10%；建议使用非程序冻存液盒货号：LH012

培养条件

生长培养基:MEM培养基；10%胎牛血清；1%双抗

生长气相:空气，95%，CO2，5%,；温度：37℃，培养箱湿度：70%-80%。

传代比例

细胞消化

1:2传代，消化1-2分钟

倍增时间

每周 2 至 3 次

产品使用

仅限于科学研究，不可作为动物或人类疾病的治疗产品使用。

注意事项

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